Aug 11, 2017 · After protein–DNA complex formation, most DNA base modifiers or excision proteins use a base flipping mechanism to access their DNA substrate ( ...
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Based on the this crystal structure, it was suggested that flipping of the target cytosine (target C) occurs via the minor groove of the DNA duplex. In uracil ...
The structure of the AlkA protein–DNA complex reveals a unique mode of binding to DNA that exposes a nucleotide substrate to the enzyme active site. The bound ...
A third reason to base flip might be simply to stabilize a protein–DNA complex, preventing linear diffusion of the protein away from a particular DNA site.
DNA base flipping, or nucleotide flipping, is a mechanism in which a single nucleotide base, or nucleobase, is rotated outside the nucleic acid double helix ...
Jul 24, 2020 · We determined DNMT1 structures in complex with preferred DNA substrates revealing that DNMT1 employs flanking sequence-dependent base flipping ...
Consistent with a base flipping mechanism, tighter binding to oligonucleotides containing destabilized target base pairs was observed. Second, total intensity ...
May 12, 2004 · DNA binding and kcat are decreased 105-fold for the Val121 → Ala mutant that has a normal circular dichroism spectrum and AdoMet affinity. The ...
Sep 26, 2022 · However, the general mechanism of the Fpg/Nei family of enzymes involves the damaged base flipping outwards into the active site pocket (59).
Apr 15, 2022 · We explore the process of base-flipping for four central bases, adenine, guanine, cytosine, and thymine, in a deoxyribonucleic acid (DNA) ...